Resolution of a paradox by native mass spectrometry: facile occupation of all four metal binding sites in the dimeric zinc sensor SmtB.
نویسندگان
چکیده
The predominant species of the cyanobacterial metalloregulatory protein SmtB as observed by ESI-MS is a dimer with all four zinc binding sites occupied.
منابع مشابه
Comparative Analysis Of Heavy-Metal Ion Sensing Mechanisms With Transcription Factors, Smtbs, From Freshwater Synechococcus Sp. PCC 7942, And Marine Synechococcus Sp. PCC 7002: Evolutionary And Structural Aspects
Metal-responsive transcription factors play a crucial role in the metal ion homeostasis with both cellular metabolism and environmental metal availability. In present work, we studied structural and evolutionary features of a transcription repressor, SmtB from freshwater Synechococcus sp. PCC 7942 and its homologs from other cyanobacteria in detail. We mined putative SmtB-like zinc sensors from...
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عنوان ژورنال:
- Chemical communications
دوره 49 8 شماره
صفحات -
تاریخ انتشار 2013